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Phospho-KIF22 (Ser427) monoclonal antibody MB11819
  • Western blot analysis of Phospho-KIF22 in A549, HL-60, U2OS lysates using Phospho-KIF22 antibody.
Product NamePhospho-KIF22 (Ser427) monoclonal antibody
Catalog No.MB11819
Swiss-ProtQ14807
Host Rabbit
ReactivityHuman
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 73 kDa; Observed MW: 73 kDa
Alternative NameKIF22; KID; KNSL4; Kinesin-like protein KIF22; Kinesin-like DNA-binding protein; Kinesin-like protein 4
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-KIF22 (Ser427) monoclonal antibody
Background :
The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser427 of human KIF22
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Phospho-KIF22 in A549, HL-60, U2OS lysates using Phospho-KIF22 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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