Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
Osteopontin monoclonal antibody MB11814
  • Western blot analysis of Osteopontin in Hela, CHO-K1, C6, Jurkat lysates using Osteopontin antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Osteopontin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameOsteopontin monoclonal antibody
Catalog No.MB11814
Swiss-ProtP10451
Host Rabbit
ReactivityHuman,Hamster,Rat
ApplicationsWB,IHC-P
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100
BiowMWCalculated MW: 35 kDa; Observed MW: 35-100 kDa
Alternative NameSPP1; BNSP; OPN; PSEC0156; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
Browse similar products>>
Size Price
50ul $258
100ul $398
Add to cart My orders
Product Name :
Osteopontin monoclonal antibody
Background :
osteopontin Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. Ligand for integrin alpha-V/beta-3. 4 isoforms of the human protein are produced by alternative splicing.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Osteopontin
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Osteopontin in Hela, CHO-K1, C6, Jurkat lysates using Osteopontin antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Osteopontin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER