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Wnt5a monoclonal antibody MB11783
  • Western blot analysis of Wnt5a in Hela lysates using Wnt5a antibody.
  • Immunocytochemistry analysis of Wnt5a in hela using Wnt5a antibody,and DAPI
Product NameWnt5a monoclonal antibody
Catalog No.MB11783
Swiss-ProtP41221
Host Rabbit
ReactivityHuman
ApplicationsWB,ICC/IF
Application_allWB: 1/500-1/1000 IF: 1/50-1/200
BiowMWCalculated MW: 42 kDa; Observed MW: 45 kDa
Alternative NameWNT5A; Protein Wnt-5a
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Wnt5a monoclonal antibody
Background :
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Wnt5a
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Wnt5a in Hela lysates using Wnt5a antibody.
  • Immunocytochemistry analysis of Wnt5a in hela using Wnt5a antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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