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Topoisomerase I monoclonal antibody MB11707
  • Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.
  • Immunocytochemistry analysis of Topoisomerase I in Hela using Topoisomerase I antibody,and DAPI
  • Immunocytochemistry analysis of Topoisomerase I in Hela using Topoisomerase I antibody,and DAPI
Product NameTopoisomerase I monoclonal antibody
Catalog No.MB11707
Swiss-ProtP11387
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 91 kDa; Observed MW: 91 kDa
Alternative NameTOP1; DNA topoisomerase 1; DNA topoisomerase I
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Topoisomerase I monoclonal antibody
Background :
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Topoisomerase I
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.
  • Immunocytochemistry analysis of Topoisomerase I in Hela using Topoisomerase I antibody,and DAPI
  • Immunocytochemistry analysis of Topoisomerase I in Hela using Topoisomerase I antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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