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SMYD3 monoclonal antibody MB11613
  • Immunocytochemistry analysis of SMYD3 in K562 using SMYD3 antibody,and DAPI.
  • Western blot analysis of SMYD3 in Jurkat, Hela lysates using SMYD3 antibody.
  • Western blot analysis of SMYD3 in Jurkat, Hela lysates using SMYD3 antibody.
Product NameSMYD3 monoclonal antibody
Catalog No.MB11613
Swiss-ProtQ9H7B4
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 49 kDa; Observed MW: 49 kDa
Alternative NameKMT3E; ZMYND1; ZNFN3A1; bA74P14.1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
SMYD3 monoclonal antibody
Background :
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human SMYD3
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of SMYD3 in K562 using SMYD3 antibody,and DAPI.
  • Western blot analysis of SMYD3 in Jurkat, Hela lysates using SMYD3 antibody.
  • Western blot analysis of SMYD3 in Jurkat, Hela lysates using SMYD3 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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