Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
SAFB1 monoclonal antibody MB11563
  • Immunocytochemistry analysis of SAFB1 in 293 using SAFB1 antibody,and DAPI.
  • Western blot analysis of SAFB in K562, 3T3, Hela lysates using SAFB antibody.
  • Western blot analysis of SAFB in K562, 3T3, Hela lysates using SAFB antibody.
Product NameSAFB1 monoclonal antibody
Catalog No.MB11563
Swiss-ProtQ15424
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 103 kDa; Observed MW: 150 kDa
Alternative NameSAFB; HAP; HET; SAFB1; Scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
Browse similar products>>
Size Price
50ul $258
100ul $398
Add to cart My orders
Product Name :
SAFB1 monoclonal antibody
Background :
This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human SAFB
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of SAFB1 in 293 using SAFB1 antibody,and DAPI.
  • Western blot analysis of SAFB in K562, 3T3, Hela lysates using SAFB antibody.
  • Western blot analysis of SAFB in K562, 3T3, Hela lysates using SAFB antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER