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RENT1 monoclonal antibody MB11529
  • Western blot analysis of RENT1 / hUPF1 in Jurkat, Hela lysates using RENT1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using RENT1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using RENT1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameRENT1 monoclonal antibody
Catalog No.MB11529
Swiss-ProtQ92900
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 124 kDa; Observed MW: 124 kDa
Alternative NameUPF1; KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
RENT1 monoclonal antibody
Background :
Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs).
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human RENT1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of RENT1 / hUPF1 in Jurkat, Hela lysates using RENT1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using RENT1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using RENT1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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