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Phospho-Rb2 p130 (Ser952) monoclonal antibody MB11522
  • Western blot analysis of Rb2 p130 in K562, Hela lysates using Phospho-Rb2 p130 antibody.
  • Immunocytochemistry analysis of Rb2 p130 in Hela using Rb2 p130 antibody,and DAPI
  • Immunocytochemistry analysis of Rb2 p130 in Hela using Rb2 p130 antibody,and DAPI
Product NamePhospho-Rb2 p130 (Ser952) monoclonal antibody
Catalog No.MB11522
Swiss-ProtQ08999
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 128 kDa; Observed MW: 128 kDa
Alternative NameRb2; P130
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-Rb2 p130 (Ser952) monoclonal antibody
Background :
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Rb2 p130 in K562, Hela lysates using Phospho-Rb2 p130 antibody.
  • Immunocytochemistry analysis of Rb2 p130 in Hela using Rb2 p130 antibody,and DAPI
  • Immunocytochemistry analysis of Rb2 p130 in Hela using Rb2 p130 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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