Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
Pyruvate Dehydrogenase E1 alpha monoclonal antibody MB11488
  • Western blot analysis of Pyruvate Dehydrogenase E1alpha subunit in C6, 3T3 lysates using Pyruvate Dehydrogenase E1 alpha antibody.
  • Immunocytochemistry analysis of Pyruvate Dehydrogenase E1alpha subunit in Hela using Pyruvate Dehydrogenase E1alpha subunit antibody,and DAPI
Product NamePyruvate Dehydrogenase E1 alpha monoclonal antibody
Catalog No.MB11488
Swiss-ProtP08559
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,ICC/IF,IP
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 43 kDa; Observed MW: 43 kDa
Alternative Namemitochondrial; ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex; E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha; Pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochondrial; somatic form.
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
Browse similar products>>
Size Price
50ul $258
100ul $398
Add to cart My orders
Product Name :
Pyruvate Dehydrogenase E1 alpha monoclonal antibody
Background :
The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). Catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Pyruvate Dehydrogenase E1-alpha subunit
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Pyruvate Dehydrogenase E1alpha subunit in C6, 3T3 lysates using Pyruvate Dehydrogenase E1 alpha antibody.
  • Immunocytochemistry analysis of Pyruvate Dehydrogenase E1alpha subunit in Hela using Pyruvate Dehydrogenase E1alpha subunit antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER