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PUS1 monoclonal antibody MB11485
  • Western blot analysis of PUS1 in Jurkat, C6, CHO-K1, Hela lysates using PUS1 antibody.
  • Immunocytochemistry analysis of PUS1 in MCF-7 using PUS1 antibody,and DAPI
Product NamePUS1 monoclonal antibody
Catalog No.MB11485
Swiss-ProtQ9Y606
Host Rabbit
ReactivityHuman,Hamster,Rat
ApplicationsWB,ICC/IF,IP
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 47 kDa; Observed MW: 44,47 kDa
Alternative NameMLASA1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
PUS1 monoclonal antibody
Background :
Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 RNA.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human PUS1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of PUS1 in Jurkat, C6, CHO-K1, Hela lysates using PUS1 antibody.
  • Immunocytochemistry analysis of PUS1 in MCF-7 using PUS1 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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