Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
Phospho-PRC1 (Thr481) monoclonal antibody MB11450
  • Western blot analysis of Phospho-PRC1 in Hela, Jurkat lysates using Phospho-PRC1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRC1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRC1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NamePhospho-PRC1 (Thr481) monoclonal antibody
Catalog No.MB11450
Swiss-ProtO43663
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 72 kDa; Observed MW: 72 kDa
Alternative NameProtein regulator of cytokinesis 1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
Browse similar products>>
Size Price
50ul $258
100ul $398
Add to cart My orders
Product Name :
Phospho-PRC1 (Thr481) monoclonal antibody
Background :
Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Thr481 of human PRC1
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Phospho-PRC1 in Hela, Jurkat lysates using Phospho-PRC1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRC1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRC1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER