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RNA Polymerases I/II/III Subunit ABC1 monoclonal antibody MB11439
  • Western blot analysis of POLR2E in K562, C6, 3T3, Hela lysates using RNA Polymerases I/II/III Subunit ABC1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer using POLR2E antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameRNA Polymerases I/II/III Subunit ABC1 monoclonal antibody
Catalog No.MB11439
Swiss-ProtP19388
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100
BiowMWCalculated MW: 25 kDa; Observed MW: 25 kDa
Alternative NameRPB5; XAP4; RPABC1; hRPB25; hsRPB5
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
RNA Polymerases I/II/III Subunit ABC1 monoclonal antibody
Background :
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process .
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human POLR2E
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of POLR2E in K562, C6, 3T3, Hela lysates using RNA Polymerases I/II/III Subunit ABC1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer using POLR2E antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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