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Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody MB11368
  • Western blot analysis of PAK1 /PAK2 /PAK3 in C6, 3T3, Hela lysates using Phospho-PAK1/2/3 antibody.
  • Immunocytochemistry analysis of PAK1 /PAK2 /PAK3 in Hela using PAK1 /PAK2 /PAK3 antibody,and DAPI
  • Immunocytochemistry analysis of PAK1 /PAK2 /PAK3 in Hela using PAK1 /PAK2 /PAK3 antibody,and DAPI
Product NamePhospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody
Catalog No.MB11368
Swiss-ProtO75914
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-F,IHC-P,ICC/IF,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 62 kDa; Observed MW: 62 kDa
Alternative NamePAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody
Background :
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of PAK1 /PAK2 /PAK3 in C6, 3T3, Hela lysates using Phospho-PAK1/2/3 antibody.
  • Immunocytochemistry analysis of PAK1 /PAK2 /PAK3 in Hela using PAK1 /PAK2 /PAK3 antibody,and DAPI
  • Immunocytochemistry analysis of PAK1 /PAK2 /PAK3 in Hela using PAK1 /PAK2 /PAK3 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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