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Phospho-p27 Kip 1 (Ser10) monoclonal antibody MB11350
  • Western blot analysis of Phospho-p27 KIP 1 in Hela, 3T3 lysates using Phospho-p27 Kip 1 antibody.
  • Western blot analysis of Phospho-p27 KIP 1 in C6 lysates using Phospho-p27 KIP 1 antibody.
  • Western blot analysis of Phospho-p27 KIP 1 in C6 lysates using Phospho-p27 KIP 1 antibody.
Product NamePhospho-p27 Kip 1 (Ser10) monoclonal antibody
Catalog No.MB11350
Swiss-ProtP46527
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 22 kDa; Observed MW: 27 kDa
Alternative NameCDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-p27 Kip 1 (Ser10) monoclonal antibody
Background :
The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Phospho-p27 KIP 1 in Hela, 3T3 lysates using Phospho-p27 Kip 1 antibody.
  • Western blot analysis of Phospho-p27 KIP 1 in C6 lysates using Phospho-p27 KIP 1 antibody.
  • Western blot analysis of Phospho-p27 KIP 1 in C6 lysates using Phospho-p27 KIP 1 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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