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NF-KB p100 monoclonal antibody MB11304
  • Western blot analysis of NF-KB p100/NF-KB2 in K562, Hela lysates using NF-KB p100 antibody.
  • Immunocytochemistry analysis of NF-KB p100/NF-KB2 in Hela using NF-KB p100/NF-KB2 antibody,and DAPI
Product NameNF-KB p100 monoclonal antibody
Catalog No.MB11304
Swiss-ProtQ00653
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,ICC/IF,IP
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 97 kDa; Observed MW: 120 kDa
Alternative NameNFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
NF-KB p100 monoclonal antibody
Background :
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human NFkB p100/NFKB2
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of NF-KB p100/NF-KB2 in K562, Hela lysates using NF-KB p100 antibody.
  • Immunocytochemistry analysis of NF-KB p100/NF-KB2 in Hela using NF-KB p100/NF-KB2 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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