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NCK1 monoclonal antibody MB11271
  • Immunocytochemistry analysis of NCK1 in Hela using NCK1 antibody,and DAPI.
  • Western blot analysis of Nck in K562, rat Brain, C6, 3T3, Hela lysates using Nck antibody.
  • Western blot analysis of Nck in K562, rat Brain, C6, 3T3, Hela lysates using Nck antibody.
Product NameNCK1 monoclonal antibody
Catalog No.MB11271
Swiss-ProtP16333
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-F,IHC-P,ICC/IF,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 43 kDa; Observed MW: 43 kDa
Alternative NameNCK; nck-1; NCKalpha
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
NCK1 monoclonal antibody
Background :
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Nck
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of NCK1 in Hela using NCK1 antibody,and DAPI.
  • Western blot analysis of Nck in K562, rat Brain, C6, 3T3, Hela lysates using Nck antibody.
  • Western blot analysis of Nck in K562, rat Brain, C6, 3T3, Hela lysates using Nck antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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