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MCM7 monoclonal antibody MB11195
  • Western blot analysis of MCM7 in 3T3 lysates using MCM7 antibody.
  • Western blot analysis of MCM7 in K562, C6, Hela lysates using MCM7 antibody.
  • Western blot analysis of MCM7 in K562, C6, Hela lysates using MCM7 antibody.
Product NameMCM7 monoclonal antibody
Catalog No.MB11195
Swiss-ProtP33993
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 81 kDa; Observed MW: 81 kDa
Alternative NameMCM7; CDC47; MCM2; DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
MCM7 monoclonal antibody
Background :
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human MCM7
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of MCM7 in 3T3 lysates using MCM7 antibody.
  • Western blot analysis of MCM7 in K562, C6, Hela lysates using MCM7 antibody.
  • Western blot analysis of MCM7 in K562, C6, Hela lysates using MCM7 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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