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HNF 4 alpha monoclonal antibody MB11044
  • Western blot analysis of HNF4alpha in Raw264.7 lysates using HNF 4 alpha antibody.
Product NameHNF 4 alpha monoclonal antibody
Catalog No.MB11044
Swiss-ProtP49698
Host Rabbit
ReactivityMouse
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 53 kDa; Observed MW: 53 kDa
Alternative NameHnf4; HNF-4; MODY1; Nr2a1; Tcf14; TCF-14; Hnf4alpha
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
HNF 4 alpha monoclonal antibody
Background :
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and inTine.MiscellaneousBinds fatty acids.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of mouse HNF-4-alpha
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of HNF4alpha in Raw264.7 lysates using HNF 4 alpha antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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