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Histone H1.3 monoclonal antibody MB11027
  • Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates using Histone H1.3 antibody.
  • Immunocytochemistry analysis of Histone H1.3 in Hela using Histone H1.3 antibody,and DAPI
  • Immunocytochemistry analysis of Histone H1.3 in Hela using Histone H1.3 antibody,and DAPI
Product NameHistone H1.3 monoclonal antibody
Catalog No.MB11027
Swiss-ProtP16402
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 22 kDa; Observed MW: 30 kDa
Alternative NameHistone H1c; Histone H1s-2
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Histone H1.3 monoclonal antibody
Background :
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation .
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Histone H1.3
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates using Histone H1.3 antibody.
  • Immunocytochemistry analysis of Histone H1.3 in Hela using Histone H1.3 antibody,and DAPI
  • Immunocytochemistry analysis of Histone H1.3 in Hela using Histone H1.3 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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