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Phospho-Histone H2A.X (Ser139) monoclonal antibody MB10958
  • Western blot analysis of gamma H2A.X in HepG2 lysates using Phospho-Histone H2A.X antibody.
  • Western blot analysis of gamma H2A.X in Jurkat, THP-1, C6, 3T3 lysates using gamma H2A.X antibody.
Product NamePhospho-Histone H2A.X (Ser139) monoclonal antibody
Catalog No.MB10958
Swiss-ProtP16104
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IP
Application_allWB: 1/500-1/1000 IP: 1/20
BiowMWCalculated MW: 15 kDa; Observed MW: 15 kDa
Alternative NameH2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-Histone H2A.X (Ser139) monoclonal antibody
Background :
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser139 of human gamma H2A.X
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of gamma H2A.X in HepG2 lysates using Phospho-Histone H2A.X antibody.
  • Western blot analysis of gamma H2A.X in Jurkat, THP-1, C6, 3T3 lysates using gamma H2A.X antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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