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Phospho-FOXO3A (Ser253) monoclonal antibody MB10933
  • Western blot analysis of Phospho-FOXO3A in 3T3 lysates using Phospho-FOXO3A antibody.
  • Western blot analysis of Phospho-FOXO3A in K562, C6 lysates using Phospho-FOXO3A antibody.
Product NamePhospho-FOXO3A (Ser253) monoclonal antibody
Catalog No.MB10933
Swiss-ProtO43524
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 71 kDa; Observed MW: 82 kDa
Alternative NameFOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-FOXO3A (Ser253) monoclonal antibody
Background :
FoxO3a is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser253 of human FOXO3A
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Phospho-FOXO3A in 3T3 lysates using Phospho-FOXO3A antibody.
  • Western blot analysis of Phospho-FOXO3A in K562, C6 lysates using Phospho-FOXO3A antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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