Product Name :
Phospho-Cytokeratin 8 (Ser23) monoclonal antibody Background :
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. K8 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IgG Immunogen :
A synthetic phosphopeptide corresponding to residues surrounding Ser23 of human Cytokeratin 8 Conjugate :
Unconjugated Modification :
Phosphorylated

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  Western blot analysis of Phospho-Cytokeratin 8 in K562, Hela lysates using Phospho-Cytokeratin 8 antibody.
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  Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Cytokeratin 8 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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  Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Cytokeratin 8 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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