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Cytochrome P450 1B1 monoclonal antibody MB10796
  • Western blot analysis of CYP1B1 in rat Brain lysates using Cytochrome P450 1B1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CYP1B1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameCytochrome P450 1B1 monoclonal antibody
Catalog No.MB10796
Swiss-ProtQ16678
Host Rabbit
ReactivityHuman,Rat
ApplicationsWB,IHC-P
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100
BiowMWCalculated MW: 61 kDa; Observed MW: 61 kDa
Alternative NameCYP1B1; Cytochrome P450 1B1; CYPIB1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Cytochrome P450 1B1 monoclonal antibody
Background :
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human CYP1B1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of CYP1B1 in rat Brain lysates using Cytochrome P450 1B1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CYP1B1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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