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Creatine Kinase B type monoclonal antibody MB10762
  • Immunohistochemistry analysis of paraffin-embedded mouse colon using Creatine Kinase B type antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot analysis of Creatine kinase B type in K562, rat Brain, C6, 3T3 lysates using Creatine kinase B type antibody.
Product NameCreatine Kinase B type monoclonal antibody
Catalog No.MB10762
Swiss-ProtP12277
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 43 kDa; Observed MW: 43 kDa
Alternative NameBCK; Ckb; CKBB; HEL 211; HEL S 29
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Creatine Kinase B type monoclonal antibody
Background :
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Creatine kinase B type
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunohistochemistry analysis of paraffin-embedded mouse colon using Creatine Kinase B type antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot analysis of Creatine kinase B type in K562, rat Brain, C6, 3T3 lysates using Creatine kinase B type antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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