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Cleaved-Caspase 3 p12 monoclonal antibody MB10661
  • Western blot analysis of Caspase3 p12 in K562, 3T3, Hela lysates using Cleaved-Caspase 3 p12 antibody.
Product NameCleaved-Caspase 3 p12 monoclonal antibody
Catalog No.MB10661
Swiss-ProtP42574
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,ICC/IF
Application_allWB: 1/500-1/1000 IF: 1/50-1/200
BiowMWCalculated MW: 32 kDa; Observed MW: 32,12 kDa
Alternative NameCASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationCleaved
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Size Price
50ul $258
100ul $398
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Product Name :
Cleaved-Caspase 3 p12 monoclonal antibody
Background :
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human Caspase-3 p12
Conjugate :
Unconjugated
Modification :
Cleaved
  • Western blot analysis of Caspase3 p12 in K562, 3T3, Hela lysates using Cleaved-Caspase 3 p12 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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