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BMP4 monoclonal antibody MB10628
  • Immunocytochemistry analysis of BMP4 in HEPG2 using BMP4 antibody,and DAPI.
  • Western blot analysis of BMP4 in K562, Hela lysates using BMP4 antibody.
Product NameBMP4 monoclonal antibody
Catalog No.MB10628
Swiss-ProtP12644
Host Rabbit
ReactivityHuman
ApplicationsWB,ICC/IF,IP
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 47 kDa; Observed MW: 47 kDa
Alternative NameBMP4; BMP2B; DVR4; Bone morphogenetic protein 4; BMP-4; Bone morphogenetic protein 2B; BMP-2B
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
BMP4 monoclonal antibody
Background :
Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation. BMPs belongs to the TGF-β superfamily, playing many diverse functions during development. BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human BMP4
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of BMP4 in HEPG2 using BMP4 antibody,and DAPI.
  • Western blot analysis of BMP4 in K562, Hela lysates using BMP4 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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