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Actin Related Protein 3 monoclonal antibody MB10570
  • Immunohistochemistry analysis of paraffin-embedded mouse colon using Actin Related Protein 3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot analysis of Arp3 in K562, rat Brain, C6, 3T3, Hela lysates using Arp3 antibody.
  • Western blot analysis of Arp3 in K562, rat Brain, C6, 3T3, Hela lysates using Arp3 antibody.
Product NameActin Related Protein 3 monoclonal antibody
Catalog No.MB10570
Swiss-ProtP61158
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 47 kDa; Observed MW: 47 kDa
Alternative NameACTR3; ARP3; Actin-related protein 3; Actin-like protein 3
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Actin Related Protein 3 monoclonal antibody
Background :
The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Arp3
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunohistochemistry analysis of paraffin-embedded mouse colon using Actin Related Protein 3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot analysis of Arp3 in K562, rat Brain, C6, 3T3, Hela lysates using Arp3 antibody.
  • Western blot analysis of Arp3 in K562, rat Brain, C6, 3T3, Hela lysates using Arp3 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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