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eIF5A monoclonal antibody MB10470
  • Western blot analysis of eIF5A in Jurkat lysates using eIF5A antibody.
  • Western blot analysis of eIF5A in Jurkat, THP-1, C6, 3T3, Hela lysates using eIF5A antibody.
  • Western blot analysis of eIF5A in Jurkat, THP-1, C6, 3T3, Hela lysates using eIF5A antibody.
Product NameeIF5A monoclonal antibody
Catalog No.MB10470
Swiss-ProtP63241
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 17 kDa; Observed MW: 17 kDa
Alternative NameEIF-5A; EIF5A1; eIF5AI; Eukaryotic initiation factor 5A
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
eIF5A monoclonal antibody
Background :
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human eIF5A
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of eIF5A in Jurkat lysates using eIF5A antibody.
  • Western blot analysis of eIF5A in Jurkat, THP-1, C6, 3T3, Hela lysates using eIF5A antibody.
  • Western blot analysis of eIF5A in Jurkat, THP-1, C6, 3T3, Hela lysates using eIF5A antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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