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Bcl2 monoclonal antibody MB10443
  • Western blot analysis of Bcl2 in MCF-7 lysates using Bcl2 antibody.
  • Western blot analysis of Bcl2 in K562, CHO-K1, 3T3, Hela lysates using Bcl2 antibody.
  • Western blot analysis of Bcl2 in K562, CHO-K1, 3T3, Hela lysates using Bcl2 antibody.
Product NameBcl2 monoclonal antibody
Catalog No.MB10443
Swiss-ProtP10415
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,IHC-P
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100
BiowMWCalculated MW: 26 kDa; Observed MW: 26 kDa
Alternative NameBCL2; Apoptosis regulator Bcl-2
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Bcl2 monoclonal antibody
Background :
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human Bcl-2
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Bcl2 in MCF-7 lysates using Bcl2 antibody.
  • Western blot analysis of Bcl2 in K562, CHO-K1, 3T3, Hela lysates using Bcl2 antibody.
  • Western blot analysis of Bcl2 in K562, CHO-K1, 3T3, Hela lysates using Bcl2 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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