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ROCK2 monoclonal antibody MB10294
  • Western blot analysis of ROCK2 in Hela, rat Brain, K562 lysates using ROCK2 antibody.
  • Immunocytochemistry analysis of ROCK2 in Hela using ROCK2 antibody,and DAPI
Product NameROCK2 monoclonal antibody
Catalog No.MB10294
Swiss-ProtO75116
Host Rabbit
ReactivityHuman,Rat
ApplicationsWB,ICC/IF
Application_allWB: 1/500-1/1000 IF: 1/50-1/200
BiowMWCalculated MW: 161 kDa; Observed MW: 161 kDa
Alternative Namep164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
ROCK2 monoclonal antibody
Background :
Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human ROCK2
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of ROCK2 in Hela, rat Brain, K562 lysates using ROCK2 antibody.
  • Immunocytochemistry analysis of ROCK2 in Hela using ROCK2 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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