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DNA Nucleotidylexotransferase monoclonal antibody MB10241
  • Western blot analysis of TdT in Jurkat lysates using DNA Nucleotidylexotransferase antibody.
Product NameDNA Nucleotidylexotransferase monoclonal antibody
Catalog No.MB10241
Swiss-ProtP04053
Host Rabbit
ReactivityHuman
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 59 kDa; Observed MW: 59 kDa
Alternative NameDNA nucleotidylexotransferase; DNTT; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
DNA Nucleotidylexotransferase monoclonal antibody
Background :
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human TdT
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of TdT in Jurkat lysates using DNA Nucleotidylexotransferase antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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