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CDKN2A/p16INK4a polyclonal antibody BZ16991
  • Immunocytochemistry analysis of CDKN2A in 293 using CDKN2A antibody,and DAPI.
  • Western blot analysis of p16 INK4A in 293 lysates using p16 INK4A antibody.
Product NameCDKN2A/p16INK4a polyclonal antibody
Catalog No.BZ16991
Swiss-ProtP42771
Host Rabbit
ReactivityHuman
ApplicationsFC,ICC/IF,IP,WB
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
BiowMWCalculated MW: 17 kDa; Observed MW: 17 kDa
Alternative NameCDKN2A; CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A; isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
CDKN2A/p16INK4a polyclonal antibody
Background :
p16-INK4A a cell-cycle regulatory protein that Interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6. Four alternatively spliced isoforms have been reported.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human CDKN2A/p16INK4a
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of CDKN2A in 293 using CDKN2A antibody,and DAPI.
  • Western blot analysis of p16 INK4A in 293 lysates using p16 INK4A antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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