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Smad2/3 polyclonal antibody BZ16985
  • Immunocytochemistry analysis of Smad2/3 in HT-1080 using Smad2/3 antibody,and DAPI.
  • Western blot analysis of Smad2/3 in Hela, 293, C6, C2C12 lysates using Smad2/3 antibody.
Product NameSmad2/3 polyclonal antibody
Catalog No.BZ16985
Swiss-ProtQ15796
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,ICC/IF,FC,IP,ChIP
Application_allWB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100 ChIP: 1/20
BiowMWCalculated MW: 52 kDa; Observed MW: 52,60 kDa
Alternative NameSMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Smad2/3 polyclonal antibody
Background :
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human Smad2
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunocytochemistry analysis of Smad2/3 in HT-1080 using Smad2/3 antibody,and DAPI.
  • Western blot analysis of Smad2/3 in Hela, 293, C6, C2C12 lysates using Smad2/3 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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