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ATP6V1E1 polyclonal antibody BZ16983
  • Western blot analysis of ATP6V1E1 in 3T3, Hela, 293 lysates using ATP6V1E1 antibody.
  • Immunocytochemistry analysis of ATP6V1E1 in Hela using ATP6V1E1 antibody,and DAPI
  • Immunocytochemistry analysis of ATP6V1E1 in Hela using ATP6V1E1 antibody,and DAPI
Product NameATP6V1E1 polyclonal antibody
Catalog No.BZ16983
Swiss-ProtP36543
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,IHC-F,IHC-P,ICC/IF,FC,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
BiowMWCalculated MW: 26 kDa; Observed MW: 26 kDa
Alternative NameV-ATPase subunit E 1; p31
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
ATP6V1E1 polyclonal antibody
Background :
Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Recombinant protein of human ATP6V1E1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of ATP6V1E1 in 3T3, Hela, 293 lysates using ATP6V1E1 antibody.
  • Immunocytochemistry analysis of ATP6V1E1 in Hela using ATP6V1E1 antibody,and DAPI
  • Immunocytochemistry analysis of ATP6V1E1 in Hela using ATP6V1E1 antibody,and DAPI
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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