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Cyclin D1 polyclonal antibody BZ16976
  • Western blot analysis of Cyclin D1 in 3T3 lysates using Cyclin D1 antibody.
  • Western blot analysis of Cyclin D1 in MCF-7, C6, PC-12 lysates using Cyclin D1 antibody.
  • Western blot analysis of Cyclin D1 in MCF-7, C6, PC-12 lysates using Cyclin D1 antibody.
Product NameCyclin D1 polyclonal antibody
Catalog No.BZ16976
Swiss-ProtP24385
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 34 kDa; Observed MW: 34 kDa
Alternative NameCCND1; BCL1; D11S287E; PRAD1; U21B31; Cyclin D1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Cyclin D1 polyclonal antibody
Background :
Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.
Product :
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human Cyclin D1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Cyclin D1 in 3T3 lysates using Cyclin D1 antibody.
  • Western blot analysis of Cyclin D1 in MCF-7, C6, PC-12 lysates using Cyclin D1 antibody.
  • Western blot analysis of Cyclin D1 in MCF-7, C6, PC-12 lysates using Cyclin D1 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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