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DNA Ligase IV polyclonal antibody BZ16904
  • Western blot analysis of DNA Ligase IV in Ramos lysates using DNA Ligase IV antibody.
Product NameDNA Ligase IV polyclonal antibody
Catalog No.BZ16904
Swiss-ProtP49917
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 104 kDa; Observed MW: 104 kDa
Alternative NameLIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
DNA Ligase IV polyclonal antibody
Background :
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human DNA Ligase IV
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of DNA Ligase IV in Ramos lysates using DNA Ligase IV antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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