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GATA1 polyclonal antibody BZ16805
  • Western blot analysis of GATA1 in K562 lysates using GATA1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using GATA1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameGATA1 polyclonal antibody
Catalog No.BZ16805
Swiss-ProtP15976
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-P,ICC/IF,IP,ChIP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
BiowMWCalculated MW: 43 kDa; Observed MW: 43 kDa
Alternative NameGATA1; ERYF1; GF1; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
GATA1 polyclonal antibody
Background :
GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development. Mutations in GATA-1 have been linked to many human diseases, including acute megakaryoblastic leukemia in Down syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human GATA1
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of GATA1 in K562 lysates using GATA1 antibody.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using GATA1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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