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Phospho-CDK2 (Tyr15) polyclonal antibody BZ16689
  • Western blot analysis of Phospho-CDK2 in HeLa treated with AP; HeLa lysates using Phospho-CDK2 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human bladder cancer using Phospho-CDK2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NamePhospho-CDK2 (Tyr15) polyclonal antibody
Catalog No.BZ16689
Swiss-ProtP24941
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
BiowMWCalculated MW: 34 kDa; Observed MW: 34 kDa
Alternative NameCDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-CDK2 (Tyr15) polyclonal antibody
Background :
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human CDK2
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Phospho-CDK2 in HeLa treated with AP; HeLa lysates using Phospho-CDK2 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human bladder cancer using Phospho-CDK2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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