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Rb polyclonal antibody BZ16670
  • Western blot analysis of Retinoblastoma in Jurkat lysates; MCF-7 lysates using Rb antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameRb polyclonal antibody
Catalog No.BZ16670
Swiss-ProtP06400
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,IHC-P,ICC/IF,IP
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
BiowMWCalculated MW: 106 kDa; Observed MW: 106 kDa
Alternative NameRB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Rb polyclonal antibody
Background :
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human Retinoblastoma
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Retinoblastoma in Jurkat lysates; MCF-7 lysates using Rb antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Retinoblastoma antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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