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ATG7 polyclonal antibody BZ16669
  • Western blot analysis of ATG7 in HepG2 lysates using ATG7 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameATG7 polyclonal antibody
Catalog No.BZ16669
Swiss-ProtO95352
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-P,ICC/IF,IP,FC
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
BiowMWCalculated MW: 78 kDa; Observed MW: 78 kDa
Alternative NamehAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
ATG7 polyclonal antibody
Background :
The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human ATG7
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of ATG7 in HepG2 lysates using ATG7 antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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