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CD3 epsilon polyclonal antibody BZ16651
  • Western blot analysis of CD3 epsilon in Jurkat lysates using CD3 epsilon antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human colon cancer using CD3 epsilon antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameCD3 epsilon polyclonal antibody
Catalog No.BZ16651
Swiss-ProtP07766
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC-P,IP,FC
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 FC: 1/50-1/100
BiowMWCalculated MW: 23 kDa; Observed MW: 23 kDa
Alternative NameCD3E; T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
CD3 epsilon polyclonal antibody
Background :
The CD3 complex mediates signal transduction. When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery . This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human CD3 epsilon
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of CD3 epsilon in Jurkat lysates using CD3 epsilon antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human colon cancer using CD3 epsilon antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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