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Fibrillarin polyclonal antibody BZ16628
  • Western blot analysis of Fibrillarin in HepG2 lysates using Fibrillarin antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillarin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillarin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameFibrillarin polyclonal antibody
Catalog No.BZ16628
Swiss-ProtP22087
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,ICC/IF,IP,FC
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
BiowMWCalculated MW: 34 kDa; Observed MW: 34 kDa
Alternative NameFIB; FLRN; Nop1; RNU3IP1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Fibrillarin polyclonal antibody
Background :
Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human Fibrillarin
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Fibrillarin in HepG2 lysates using Fibrillarin antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillarin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillarin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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