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MonoMethyl-Histone H2B (Arg79) polyclonal antibody BZ16622
  • Western blot analysis of Histone H2B in HeLa lysates using MonoMethyl-Histone H2B antibody.
Product NameMonoMethyl-Histone H2B (Arg79) polyclonal antibody
Catalog No.BZ16622
Swiss-ProtQ16778
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 14 kDa; Observed MW: 14 kDa
Alternative NameH2BR79me; H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationMethylated
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Size Price
50ul $258
100ul $398
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Product Name :
MonoMethyl-Histone H2B (Arg79) polyclonal antibody
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human Histone H2B (mono methyl R79)
Conjugate :
Unconjugated
Modification :
Methylated
  • Western blot analysis of Histone H2B in HeLa lysates using MonoMethyl-Histone H2B antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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