C/EBP-α (M15) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
C/EBP-α (M15) polyclonal antibody Background :
C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
C/EBP-α (M15) polyclonal antibody detects endogenous levels of C/EBP-α protein. Immunogen :
Synthetic peptide, corresponding to amino acids N-terminus of Human C/EBP-α. Conjugate :
Unconjugated Modification :
Unmodified
C/EBP-α (M15) polyclonal antibody Background :
C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
C/EBP-α (M15) polyclonal antibody detects endogenous levels of C/EBP-α protein. Immunogen :
Synthetic peptide, corresponding to amino acids N-terminus of Human C/EBP-α. Conjugate :
Unconjugated Modification :
Unmodified
-
Western blot analysis of C/EBP alpha expression in mouse embryo (A), rat ovary (B), NIH3T3L1 (C), PC12 (D), Hela (E), Panc1 (F), SGC7901 (G) whole cell lysates. -
Immunohistochemical analysis of C/EBP alpha staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The endocrine disruptor diethylstilbestrol induces adipocyte differentiation and promotes obesity in mice
PMCID: Pubmed No.:22710028
miR-511 and miR-1297 Inhibit Human Lung Adenocarcinoma Cell Proliferation by Targeting Oncogene TRIB2
PMCID: Pubmed No.:23071539
TGF-β/BMP signaling pathway is involved in cerium-promoted osteogenic differentiation of mesenchymal stem cells†‡
PMCID: Pubmed No.:23150386
Let-7c inhibits A549 cell proliferation through oncogenic TRIB2 related factors
PMCID: Pubmed No.:23850892
Terbium promotes adhesion and osteogenic differentiation of mesenchymal stem cells via activation of the Smad-dependent TGF-β/BMP signaling pathway
PMCID: Pubmed No.:24585101
Curcumin regulates cell fate and metabolism by inhibiting hedgehog signaling in hepatic stellate cells
PMCID: Pubmed No.:25938627
Terbium promotes adhesion and osteogenic differentiation of mesenchymal stem cells via activation of the Smad-dependent TGF-β/BMP signaling pathway
PMCID: Pubmed No.:24585101
Curcumin regulates cell fate and metabolism by inhibiting hedgehog signaling in hepatic stellate cells
PMCID: Pubmed No.:25938627
The anti-obesity properties of the proanthocyanidin extract from the leaves of Chinese bayberry (Myrica rubra Sieb.et Zucc.)
PMCID: Pubmed No.:28828418
microRNA-451-modulated hnRNP A1 takes a part in granulocytic differentiation regulation and acute myeloid leukemia
PMCID: Pubmed No.:28903433
Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin
PMCID: Pubmed No.:33621367
Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish
PMCID: Pubmed No.:31372513
MicroRNA-128 is involved in dexamethasone-induced lipid accumulation via repressing SIRT1 expression in cultured pig preadipocytes
PMCID: Pubmed No.:30394333
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.