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NFkB-p65 (M270) polyclonal antibody BS1253
  • Western blot (WB) analysis of NFκB-p65 (M270) pAb at 1:2000 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:Hela whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:CT26 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of NFkB-P65 (M270) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of NFkB-P65 (M270) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameNFkB-p65 (M270) polyclonal antibody
Catalog No.BS1253
Swiss-ProtQ04206
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IP IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:50~200
BiowMW~ 60, 75 kDa
Alternative Namep65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $158
100ul $275
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Product Name :
NFkB-p65 (M270) polyclonal antibody
Background :
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
NFκB-p65 (M270) polyclonal antibody detects endogenous levels of NFκB-p65 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 250-300 of Human NFkB-p65.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot (WB) analysis of NFκB-p65 (M270) pAb at 1:2000 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:Hela whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:CT26 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of NFkB-P65 (M270) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of NFkB-P65 (M270) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro PMCID:    Pubmed No.:20138259 Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-kB activation PMCID:    Pubmed No.:21078355 Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-kB/COX-2 Pathway PMCID:    Pubmed No.:21779360 Nonenzymatic glycation of HDL impairs its anti-inflammatory effects in innate immunity PMCID:    Pubmed No.:21928330 Curcumin inhibits tumor proliferation induced by neutrophil elastase through the upregulation of a1-antitrypsin in lung cancer PMCID:    Pubmed No.:22507634 Effects of glutamine supplementation on the immune status in weaning piglets with intrauterine growth retardation PMCID:    Pubmed No.:22962945 Pioglitazone attenuates progression of aortic valve calcification via down-regulating receptor for advanced glycation end products. 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PMCID:    Pubmed No.:25063031 Glucose dominates the regulation of carboxylesterases induced by lipopolysaccharide or interleukin-6 in primary mouse hepatocytes PMCID:    Pubmed No.:25066929 Angiogenin Reduces Immune Inflammation via Inhibition of TANK-Binding Kinase 1 Expression in Human Corneal Fibroblast Cells PMCID:    Pubmed No.:24860242 Noscapine Increases the Sensitivity of Drug-Resistant Ovarian Cancer Cell Line SKOV3/DDP to Cisplatin by Regulating Cell Cycle and Activating Apoptotic Pathways PMCID:    Pubmed No.:25510462 The protective effect of CDDO-Me on lipopolysaccharide-induced acute lung injury in mice. PMCID:    Pubmed No.:25614226 Protective effects of protocatechuic acid on acute lung injury induced by lipopolysaccharide in mice via p38MAPK and NF-κB signal pathways PMCID:    Pubmed No.:25841318 Resveratrol inhibits inflammation and ameliorates insulin resistant endothelial dysfunction via regulation of AMPK and SIRT1 activities PMCID:    Pubmed No.:25850408 Fucoidan Suppresses Hypoxia-Induced Lymphangiogenesis and Lymphatic Metastasis in Mouse Hepatocarcinoma PMCID:    Pubmed No.:26047481 Doxycycline Protects Thymic Epithelial Cells from Mitomycin C-Mediated Apoptosis In Vitro via Trx2-NF-κB-Bcl-2/Bax Axis PMCID:    Pubmed No.:26828432 Angiogenin Reduces Immune Inflammation via Inhibition of TANK-Binding Kinase 1 Expression in Human Corneal Fibroblast Cells PMCID:    Pubmed No.:24860242 Noscapine Increases the Sensitivity of Drug-Resistant Ovarian Cancer Cell Line SKOV3/DDP to Cisplatin by Regulating Cell Cycle and Activating Apoptotic Pathways PMCID:    Pubmed No.:25510462 Doxycycline Protects Thymic Epithelial Cells from Mitomycin C-Mediated Apoptosis In Vitro via Trx2-NF-κB-Bcl-2/Bax Axis PMCID:    Pubmed No.:26828432 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 ART1 Silencing Enhances Apoptosis of Mouse CT26 Cells via the PI3K/Akt/NF-κB Pathway PMCID:    Pubmed No.:24335275 Adenosine 5′-monophosphate ameliorates d-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice PMCID:    Pubmed No.:24407238 LFG-500, a newly synthesized flavonoid, attenuates lipopolysaccharide-induced acute lung injury and inflammation in mice PMCID:    Pubmed No.:27206337 Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner PMCID:    Pubmed No.:28886428 Antitumor and anti-metastatic mechanisms of Rhizoma paridis saponins in Lewis mice PMCID:    Pubmed No.:29148169 Curcumin improves the paclitaxel‑induced apoptosis of HPV‑positive human cervical cancer cells via the NF‑κB‑p53‑caspase‑3 pathway PMCID:    Pubmed No.:25780454 Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats PMCID:    Pubmed No.:25322861 Profilin‑1 contributes to cardiac injury induced by advanced glycation end‑products in rats PMCID:    Pubmed No.:28901418 Feasibility and Mechanism Analysis of Shenfu Injection in the Treatment of Idiopathic Pulmonary Fibrosis PMCID:    Pubmed No.:34393772 Huanglianjiedu Decoction as an effective treatment for oral squamous cell carcinoma based on network pharmacology and experimental validation PMCID:    Pubmed No.:34674717 Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats PMCID:    Pubmed No.:29800538 Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation PMCID:    Pubmed No.:31572058 Magnesium isoglycyrrhizinate ameliorates doxorubicin‑induced acute cardiac and hepatic toxicity via anti‑oxidant and anti‑apoptotic mechanisms in mice PMCID:    Pubmed No.:29399108 Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice. PMCID:    Pubmed No.:36059948
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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