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Tubulin β (4D9) monoclonal antibody BS1482M
  • Western blot (WB) analysis of Tubulin β (4D9) mAb at 1:5000/1:10000/1:20000 dilution Lane1:L02 whole cell lysate(20ug) Lane2:NIH-3T3 whole cell lysate(20ug) Lane3:PC12 whole cell lysate(20ug) Lane4:The Kidney tissue lysate of Mouse(20ug)
Product NameTubulin β (4D9) monoclonal antibody
Catalog No.BS1482M
Swiss-ProtQ13509/P04350/P68371/P07437/Q13885/Q9BUF5
Host Mouse
ReactivityHuman,Mouse, Rabbit, Frog, Fish, Rat(not applicable to some rat tissue)
ApplicationsWB IHC IF IP
Application_allWB: 1:5000~20000 IHC: 1:100~500 IF: 1:100~500 IP: 1:100~500
BiowMW~ 55 kDa
Alternative NameTotal tubulin β; Tubulin beta
Purification&PurityThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $148
100ul $258
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Product Name :
Tubulin β (4D9) monoclonal antibody
Background :
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da._x000D_Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot (WB) analysis of Tubulin β (4D9) mAb at 1:5000/1:10000/1:20000 dilution Lane1:L02 whole cell lysate(20ug) Lane2:NIH-3T3 whole cell lysate(20ug) Lane3:PC12 whole cell lysate(20ug) Lane4:The Kidney tissue lysate of Mouse(20ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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