Caspase-8 (S347) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Caspase-8 (S347) polyclonal antibody Background :
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 8 (S347) polyclonal antibody detects endogenous levels of Caspase 8 protein. Immunogen :
Synthetic peptide, corresponding to Human Caspase-8. Conjugate :
Unconjugated Modification :
Unmodified
Caspase-8 (S347) polyclonal antibody Background :
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 8 (S347) polyclonal antibody detects endogenous levels of Caspase 8 protein. Immunogen :
Synthetic peptide, corresponding to Human Caspase-8. Conjugate :
Unconjugated Modification :
Unmodified
-
Western blot (WB) analysis of Caspase-8 pAb at 1:2000 dilution Lane1:PC12 whole cell lysate(30ug) Lane2:BV2 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug) Lane4:MCF-7 whole cell lysate(20ug) -
Immunohistochemistry (IHC) analyzes of Caspase-8 (S347) pAb in paraffin-embedded human lung carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of Caspase-8 (S347) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.
UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation
PMCID: Pubmed No.:21307340
Biphasic effect of EGb761 on simulated ischemia-induced rat BMSC survival in vitro and in vivo
PMCID: Pubmed No.:21439976
In vitro Anti-Angiogenesis Effects and Active Constituents of the Saponin Fraction From Gleditsia sinensis
PMCID: Pubmed No.:22505594
Induction and Mechanism of Apoptosis by Hydroxycamptothecin in Human Tenon's Capsule Fibroblasts
PMCID: Pubmed No.:22661476
MiR-320a effectively suppresses lung adenocarcinoma cell proliferation and metastasis by regulating STAT3 signals
PMCID: Pubmed No.:28106481
Fruiting body polysaccharides of Hericium erinaceus induce apoptosis in human colorectal cancer cells via ROS generation mediating caspase-9-dependent signaling pathways
PMCID: Pubmed No.:32573644
Mycobacterium tuberculosis PPE10 (Rv0442c) alters host cell apoptosis and cytokine profile via linear ubiquitin chain assembly complex HOIP-NF-κB signaling axis
PMCID: Pubmed No.:33667868
Synergistic antitumour effects of triptolide plus 10-hydroxycamptothecin onbladder cancer
PMCID: Pubmed No.:31063955
Sodium tanshinone IIA sulfonate protects ARPE-19 cells against oxidative stress by inhibiting autophagy and apoptosis
PMCID: Pubmed No.:30310136
Epidermal growth factor‐containing fibulin‐like extracellular matrix protein 1 (EFEMP1) suppressed the growth of hepatocellular carcinoma cells by promoting Semaphorin 3B(SEMA3B)
PMCID: Pubmed No.:30972979
Overexpression of HACE1 in gastric cancer inhibits tumor aggressiveness by impeding cell proliferation and migration
PMCID: Pubmed No.:29673126
Diterpene pekinenal from euphorbia pekinensis radix induced IEC-6 cells apoptosis mediated by mitochondria and death receptors
PMCID: Pubmed No.:30710624
Synergistic suppression effect on tumor growth of acute myeloid leukemia by combining cytarabine with an engineered oncolytic vaccinia virus
PMCID: Pubmed No.:30410347
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.