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FN1 monoclonal antibody MB63314
  • Western blot analysis of Fibronectin (1E2) in MCF-7 and Hela lysates using Fibronectin antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human kidney using Fibronectin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameFN1 monoclonal antibody
Catalog No.MB63314
Swiss-ProtP02751
Host Mouse
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF/ICC
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMW~ 285 kDa
Alternative NameCIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF
Purification&PurityPurified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $168
100ul $298
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Product Name :
FN1 monoclonal antibody
Background :
FN1 Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers.
Product :
Mouse IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
FN1 monoclonal antibody detects endogenous levels of FN1 protein.
Immunogen :
Synthetic Peptide of Fibronectin.
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of Fibronectin (1E2) in MCF-7 and Hela lysates using Fibronectin antibody.
  • Immunohistochemistry analysis of paraffin-embedded Human kidney using Fibronectin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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