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PERK (Phospho-Thr982) polyclonal antibody BS66100
  • Western blot analysis of PERK (pT982) expression in A375 (A), MCF7 (B), mouse testis (C) whole cell lysates.
  • Immunohistochemical analysis of PERK (pT982) staining in human lung cancer formalin fixed paraffin embedded tissue section.
Product NamePERK (Phospho-Thr982) polyclonal antibody
Catalog No.BS66100
Swiss-ProtQ9NZJ5
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB IHC
Application_allWB 1:500 - 1:1000 IHC 1:100 - 1:200
BiowMW~ 140 kDa
Alternative Namep-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylated
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50ul $228
100ul $398
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Product Name :
PERK (Phospho-Thr982) polyclonal antibody
Background :
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
PERK (Phospho-Thr980) polyclonal antibody detects endogenous levels of PERK protein only when phosphorylated at Thr980.
Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PERK.
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of PERK (pT982) expression in A375 (A), MCF7 (B), mouse testis (C) whole cell lysates.
  • Immunohistochemical analysis of PERK (pT982) staining in human lung cancer formalin fixed paraffin embedded tissue section.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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