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Recombinant Rat Insulinlike growth factor I (rRtIGF-1) PR3006
RecombinantRecombinant Rat Insulinlike growth factor I (rRtIGF-1)
Catalog No.PR3006
SourceEscherichia coli.
Molecular WeightApproximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acid residues.
Purity>97% by SDS-PAGE and HPLC analyses.
Biological ActivityMeasured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.6-3 ng/mL.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
EndotoxinLess than 1EU/μg of rRtIGF-1 as determined by LAL method.
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50µg $298
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Recombinant :
Recombinant Rat Insulinlike growth factor I (rRtIGF-1)
Source :
Escherichia coli.
Molecular Weight :
Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acid residues.
Purity :
>97% by SDS-PAGE and HPLC analyses.
Biological Activity :
Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.6-3 ng/mL.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
AA Sequence :
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
Endotoxin :
Less than 1EU/μg of rRtIGF-1 as determined by LAL method.
Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage :
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
Description :
Insulinlike growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. It shares 61% aa sequence identity with mature rat IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects. IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally.
Blocking peptide available as PR3006P
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